Enzyme Immunoassay for Detection of IgM Antibodies to Herpes Simplex Virus Type 2 (HSV-2) in Human Serum

Product Description
Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are two immunologically distinct types of HSV: Type 1 and Type 2.
HSV-1 is generally associated with oral infection and lesions above the waist, and HSV-2 is associated with genital infections and lesions below the waist.
Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivo-stomatitis, and (3) herpes sepsis, almost only found in premature newborn infants.
The HSV-2 IgM ELISA is an accurate serologic method to detect HSV-2 IgM specific-antibody in serum samples.

Principle
Purified HSV-2 antigen is coated on the surface of microwells. Diluted patient serum is added to the wells, and the HSV-2 IgM- specific antibody, if present, binds to the antigen. All unbound materials are washed away.
HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added.
The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgM- specific antibody in the sample.
The results are read by a microwell reader compared in a parallel manner with calibrator and controls.

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